Active Protein Kinase, cGMP Dependent Type II (PRKG2)
PRKGR2; cGKII; cGMP-dependent protein kinase 2
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 純度> 90%
- 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 2160 ¥ 5400 ¥ 10800 ¥ 32400 ¥ 81000
- Figure. SDS-PAGE
- Figure. Western Blot
- 通過ISO 9001、ISO 13485質量體系認證
Figure. The binding activity of PRKG2 with HSP90aA1.
Protein Kinase, cGMP Dependent Type II (PRKG2) belong to cGMP-dependent protein kinase or Protein Kinase G (PKG) which is a serine/threonine-specific protein kinase that is activated by cGMP. Two PKG genes, coding for PKG type I (PKG-I) and type II (PKG-II), have been identified in mammals. The PKG-I and PKG-II are homodimers of two identical subunits (~75kDa and ~85kDa, respectively) and share common structural features. PKG phosphorylates a number of biologically important targets and is implicated in the regulation of smooth muscle relaxation, platelet function, sperm metabolism, cell division, and nucleic acid synthesis. Besides, Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) has been identified as an interactor of PRKG2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PRKG2 and recombinant human HSP90aA1. Briefly, PRKG2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to HSP90aA1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PRKG2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of PRKG2 and HSP90aA1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|編號||適用物種：Homo sapiens (Human，人)||應用(僅供研究使用，不用于臨床診斷！)|
|RPH313Hu01||cGMP依賴性蛋白激酶Ⅱ(PRKG2)重組蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAH313Hu01||cGMP依賴性蛋白激酶Ⅱ(PRKG2)多克隆抗體||WB; IHC; ICC; IP.|
|MAH313Hu21||cGMP依賴性蛋白激酶Ⅱ(PRKG2)單克隆抗體||WB; IHC; ICC; IP.|
|SEH313Hu||cGMP依賴性蛋白激酶Ⅱ(PRKG2)檢測試劑盒(酶聯免疫吸附試驗法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PLoS One||Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788]|